TitleValidation of the Biofish-300 HIS Enzymatic Biosensor for the Detection of Histamine in Fishery Products.
Publication TypeJournal Article
Year of Publication2016
AuthorsSalleres, S, González, I, Arantzamendi, A, González, R, Maza, S, Jaureguibeitia, A, Hungerford, JM, DeWitt, CA, Benner, RA
JournalJ AOAC Int
Volume99
Issue5
Pagination1338-55
Date Published2016 Sep
ISSN1060-3271
KeywordsAnimals, Aromatic-L-Amino-Acid Decarboxylases, Biosensing Techniques, Calibration, Electrodes, Fish Products, Fisheries, Histamine
Abstract

The Biofish-300 HIS method is a simple, reliable, and specific enzymatic biosensor for the detection of histamine. This technology is highly specific and selective and allows quantification of histamine in fishery products (fresh/frozen and processed) in a short time frame (2-3 min). Histamine in raw tuna, raw mackerel, raw sardine, raw anchovy, boiled tuna, canned tuna in water, canned tuna in oil, canned mackerel in tomato sauce, canned pickled sardine, and canned salted anchovy was analyzed using a water-based extract. Matrix-specific assay procedures and calibration curves were used to enable analyses to be carried out across multiple sample types. The performance of this assay was examined using samples that were naturally contaminated (reference materials and interlaboratory studies) and spiked with histamine. All data were judged against previously established acceptance criteria. Performance measures were evaluated for linearity, selectivity, matrix, lot consistency, and robustness. Results produced in all performance measures, except robustness, were within acceptable ranges. Out-of-range robustness results reflected deviation in sample volume compared to the standard assay procedures. Positive interferences from the presence of agmatine were shown.

DOI10.5740/jaoacint.16-0180
Alternate JournalJ AOAC Int
PubMed ID27619657